Table 3.

Multivariate analysis using the 91 samples of non-cancerous liver tissue showing non-alcoholic steatohepatitis (NASH) derived from biopsy specimens of patients with morbid obesity but without hepatocellular carcinoma (HCC) (NASH-O samples) and the 22 samples of non-cancerous liver tissue showing NASH derived from partial hepatectomy specimens of patients with HCC (NASH-W samples) in the initial cohorts

Probe IDa	Variables	Odds ratiob	95% confidence interval	P valuec
cg11820154	Ballooningd	0.460	0.031–6.765	0.571
	Brunt classificatione	0.347	0.100–1.207	0.096
	DNA methylation quantification	1138.492	52.740–24,576.521	7.13 × 10−6
cg26583598	Ballooningd	0.692	0.051–9.460	0.782
	Brunt classificatione	0.286	0.090–0.915	0.035
	DNA methylation quantification	1035.707	54.365–19,731.273	3.89 × 10−6
cg18210511	Ballooningd	8.990	0.219–369.308	0.247
	Brunt classificatione	0.482	0.207–1.123	0.091
	DNA methylation quantification	248.725	30.517–2027.198	2.56 × 10−7
cg00431813	Ballooningd	5.786	0.132–252.827	0.362
	Brunt classificatione	0.686	0.275–1.714	0.420
	DNA methylation quantification	232.059	31.166–1727.915	1.05 × 10−7
cg19861632	Ballooningd	2.047	0.191–21.929	0.554
	Brunt classificatione	0.610	0.302–1.232	0.168
	DNA methylation quantification	207.072	20.883–2053.237	5.20 × 10−6
cg05861743	Ballooningd	1.491	0.133–16.687	0.746
	Brunt classificatione	0.503	0.229–1.106	0.088
	DNA methylation quantification	190.885	24.574–1482.750	5.14 × 10−7
cg09580822	Ballooningd	1.491	0.124–15.383	0.795
	Brunt classificatione	0.503	0.248–1.235	0.148
	DNA methylation quantification	190.885	23.957–1361.603	4.61 × 10−7
cg09580859	Ballooningd	9.892	0.299–327.815	0.200
	Brunt classificatione	0.506	0.226–1.133	0.098
	DNA methylation quantification	180.393	24.567–1324.612	3.27 × 10−7
cg14950303	Ballooningd	1.256	0.114–13.824	0.853
	Brunt classificatione	0.702	0.315–1.565	0.387
	DNA methylation quantification	144.050	21.556–962.621	2.92 × 10−7
cg26385097	Ballooningd	1.577	0.145–17.140	0.708
	Brunt classificatione	0.595	0.275–1.288	0.188
	DNA methylation quantification	141.119	20.394–976.513	5.30 × 10−7

^aProbe ID of the Infinium HumanMethylation450 BeadChip

^bTop 10 CpG sites showing the highest odds ratio of being NASH-W samples are summarized in this table. The results of multivariate analysis for all 21 CpG sites are shown in Additional file 1: Table S7

^cP values less than 0.05 are underlined

^dBallooning was evaluated according to the non-alcoholic fatty disease activity score (Ref. 26)

^eFibrosis stage was defined according to the Brunt classification (Ref. 27)