Table 1.

RT-PCR targets and their primers for detecting Tropheryma whipplei in this study

Target	Primer sequence (5′–3′)		Product size, bp	Limit of detection, copies/μL	Tm, °C
	Forward	Reverse
16S–23S rRNA ITS	CCGGTGACTTAACCTTTTTGGAGA	TCCCGAGGCTTATCGCAGATTG	489	190	84
rpoB	CTCGGTGTTGATGTTGATCCAA	GCACCGCAACCTCGGAGAAA	109	1830	63
hsp65	CGCGAAAGAGGTTGAGACTG	ACATCTTCAGCAATGATAAGAAGTT	357	26	82
Dig15	TGTTTTGTACTGCTTGTAACAGGATCT	GATGATAGGAGGGATAGAGCAGGA	155	599	74

ITS intergenic spacer, RT-PCR real-time PCR, T_m melting temperature