TABLE 2.
Morphological, immunohistopathological, and urinary findings
| Strain | Phenotype | Time (day p.i.) when penicillin treatment was startedb | No. of mice/total no. witha:
|
||||
|---|---|---|---|---|---|---|---|
| Glomeruli
|
Urine
|
||||||
| Occl | Lob | C3 | Protein | Hem | |||
| NZAN0 | Ska1− Emr Kanr | 7 | 1/15 | 0/15 | 1/15 | 2/15 | 0/15 |
| NZAN5 | Ska1− Emr Kanr Skc5+ | 7 | 1/25 | 0/25 | 4/25* | 7/25 | 0/25 |
| NZAN0 | Ska1− Emr Kanr | 16 | 0/14 | 0/14 | 0/14 | 1/14 | 0/14 |
| NZAN5 | Ska1− Emr Kanr Skc5+ | 16 | 0/16 | 0/16 | 1/16 | 3/16 | 0/16 |
| NZAN1 | Ska1− Emr Kanr Ska1+ | 16 | 11/36c | 0/36 | 13/36*c | 16/36**c | 0/36 |
| Uninfected mice | |||||||
| NZAN0 and NZAN5 controlsd | 0/23 | 0/23 | 0/23 | 2/23 | 1/23 | ||
| NZAN1 controlse | 1/10 | 0/10 | 1/10 | 1/10 | 0/10 | ||
For proteinuria, the minimum concentration limit was set at 1.0 g/liter; for hematuria, a hemoglobin concentration corresponding to 10 erythrocytes/μl was measured. ∗, P < 0.005; ∗∗, P < 0.01 (degree of significance for occurrence of analyzed findings, compared to the uninfected control mice in the same experiment). Abbreviations: Occl, occlusion of capillaries; Lob, lobulation; Protein, proteinuria; Hem, hematuria.
All animals were sacrificed on day 21 p.i.
Mice infected with NZAN1 had a higher occurrence of occluded capillaries (P < 0.001), C3 deposition (P < 0.01), and proteinuria (P < 0.05) than did those infected with NZAN5, treated correspondingly with penicillin.
Uninfected mice belonging to the same experiment as NZAN0- and NZAN5-infected mice.
Uninfected mice belonging to the same experiment as NZAN1-infected mice.