Table 3.

ChIP-PCR primer sets

Genes	Forward Primer	Reverse Primer	Annealing Temperature (°C)	PCR product size (bp)	TM (°C)
SALL4 P1	ATCTCTGAGGTCTTG	GCATTGCGATTTATC	51	60	74.8
SALL4 P2	GCTTGCTTATCATTTGCCATTTC	GGATTCCTGTTCACAAAGACTG	58.5	109	72.6
CD44(S) P1	GTTATTGAGGGGAAAAAGAATGAG	GGTGGGGAGTTGGTGAATC	59	65	74.1
CD44(S) P2	GCCAAATGCCAGCCCTATG	TTCCCCTCAATAACAAGTCCAC	58	103	77.9
GDF3 P1	AAGAGAGCAATTCACAC	GCCCAACAATTCAGAG	52.3	64	74.5
GDF3 P2	GCATTCAAAGCCATCG	CATCTACATAGACCAAGC	54.7	71	77.5
NANOG P1	TCCTAAACCTCAACTTTATTCC	GCCGACTTACTACATTCTTC	56	58	75.0
MEIS1 P1	AATTAGGACTGATTCAAGGG	ATCCGCTCTGTCTTCTTC	56.2	94	75.6
MEIS1 P2	TCCTTCTCTAATCTCCTTCC	AATCAGTCCTATTCCTAATCAG	57.29	106	76.0
SOX2 P1	CCCATTTATTCCCTGAC	AGTTAATAGACAACCATCC	53	50	71.1
SOX2 P2	TTGGGTCTCCTAACTTC	TTCCGCTCTCCTCTC	54.45	83	78.5

Abbreviations: P1 Primer set1, P2 Primer set2