Fig. 6.

YX-2102 enhanced Nrf2-Smad7 pathway activation in A549 cells. A549 cells pretreated with YX-2102 (5.0 µM), with or without 5 µM XL-002 (a CB2R antagonist) for 2 h, were subjected to TGF-β1 stimulation (5 ng/mL) for another 24 h. A Western blot analyses of Smad7 and Nrf2 expression. B Immunofluorescence analysis of Nrf2 (red). Nuclei were counter-stained with DAPI (blue). Scale bar = 100 μm. C Western blot analyses of Smad7 expression. D Immunohistochemical analysis of Smad7 expression in the lungs of 21-day-old rats (magnification ×200). Large boxes are magnified views of the small boxes. Scale bar = 200 μm. Data are expressed as mean ± SEM of three independent experiments