Fig. 1.

YTHDF3 is overexpressed in tumor samples from HCC patients and high expression of YTHDF3 is correlated with poor prognosis of HCC patients. a Representative immunohistochemical images were shown (left) and YTHDF3 protein expression between surrounding and carcinoma tissues of HCC patients (n = 466) (right). Scale bar 100 μm. b Representative immunohistochemical images were shown (left) and YTHDF3 protein expression between HCC patients in early and middle-late stages (n = 464) (right). Scale bar 100 μm. c Representative immunohistochemical images were shown (left) and YTHDF3 protein expression between HCC patients without or with capsular invasion (n = 466) (right). Scale bar 100 μm. d Representative immunohistochemical images were shown (left) and YTHDF3 protein expression between HCC patients without or with vascular invasion (n = 466) (right). Scale bar 100 μm. e Scatter plot analysis of tumor greatest diameters between YTHDF3 low and high-expression HCC patients. f-g The relative mRNA and protein expression of YTHDF3 in fresh tumor and surrounding tissues of HCC patients. Normalized to β-actin. h Overall survival analysis of HCC patients with low or high expression of YTHDF3 in Sun Yat-Sen Memorial Hospital (upper) and Sun Yat-Sen University Cancer Center (lower). i The background mRNA and protein expression of YTHDF3 in HCC cell lines. Normalized to β-actin. Data are shown as mean ± SD (*p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001). Arrows indicate the location of positive signal of YTHDF3 protein