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. 2022 Dec 5;15:454. doi: 10.1186/s13071-022-05502-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Expression of pyroptosis-related molecules in infected RAW264.7 cells at 24 h post-infection. a mRNA expression of pyroptosis-related molecules of NLRP3, pro-caspase-1, GSDMD, and pro-IL-1β was detected. RAW264.7 cells were infected with the RH^wx2−/−, RH, and RH^wx2+/+ strain, respectively, for 24 h, total RNA was extracted, and RT-qPCR was performed with the indicated primer. b Protein expression of pyroptosis-related molecules of NLRP3, pro-caspase-1, GSDMD, and pro-IL-1β was detected. c Analysis of grayscale scanning of image B