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. 2022 Dec 6;20:387. doi: 10.1186/s12957-022-02849-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Validation of complement proteins. A The protein expression levels of C1QB, C1QC, C4BPA, and C4BPBP in plasma sEVs from the discovery cohort. B Column chart showing the comparison of the band densities of C1QB between the control and HCC groups in the discovery cohort. C Column chart showing the comparison of the band densities of C1QC between the control and HCC groups in the discovery cohort. D Column chart showing the comparison of the band densities of C4BPA between the control and HCC groups in the discovery cohort. E Column chart showing the comparison of the band densities of C4BPB between the control and HCC groups in the discovery cohort. F The protein expression levels of C1QB, C1QC, C4BPA, and C4BPBP in plasma sEVs from the validation cohort. G Column chart showing the comparison of the band densities of C1QB between the control and HCC groups in the validation cohort. H Column chart showing the comparison of the band densities of C1QC between the control and HCC groups in the validation cohort. I Column chart showing the comparison of the band densities of C4BPA between the control and HCC groups in the validation cohort. J Column chart showing the comparison of the band densities of C4BPB between the control and HCC groups in the validation cohort. The left ordinate shows the gray value measured using ImageJ software. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001