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. 2000 Mar;68(3):1040–1047. doi: 10.1128/iai.68.3.1040-1047.2000

FIG. 1.

FIG. 1

Immunoblot analysis of E. coli lysogen containing the gene encoding the 22-kDa protein. Western blotting was performed with MAb 9E5. Lanes 1 to 3 represent CF of M. bovis BCG, a crude lysate of E. coli lysogenized with clone A1 (lysogen of M. tuberculosis PstS-3) as a negative control, and a crude lysate of E. coli lysogenized with clone C3 (lysogen of the M. bovis BCG 22-kDa protein). Positions of molecular weight markers (Rainbow markers; Amersham) are indicated at the left. The arrow indicates the presence of the 22-kDa protein of M. bovis BCG.