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. 2000 Mar;68(3):1040–1047. doi: 10.1128/iai.68.3.1040-1047.2000

FIG. 3.

FIG. 3

Electrophoresis and immunoblotting analysis of the 22-kDa protein. (A) Purification by glutathione affinity chromatography of the 22-kDa protein fused to GST. Eluates were analyzed by SDS-PAGE (15% gel) and stained with Coomassie blue. Lane 1, molecular weight markers; lane 2, purified fusion protein; lane 3, fusion protein cleaved by factor Xa. (B) Immunoblotting analysis with MAbs 9E5 (lane 1), VD12-1 (lane 2), and VIIIH-1 (lane 3) of the GST–22-kDa fusion protein following cleavage. The arrow indicates the presence of the 22-kDa protein of M. bovis BCG.