FIG. 6.

Antibody production directed against the GST–22-kDa fusion protein in DNA-vaccinated mice. Serum samples from three mice vaccinated with the empty V1Jns.tPA vector (columns 1 to 3) and five mice immunized with plasmid DNA encoding the 22-kDa protein (columns 4 to 8) of three different mouse strains (C57BL/6, C3H, and BALB/c) were tested in ELISA. Total anti-GST–22-kDa fusion protein Ig is expressed in arbitrary units (left), and levels of specific Ig isotypes are expressed as OD₄₉₂ values for a serum dilution of 1:1,600 (right).