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. 2022 Oct 26;23(12):e55648. doi: 10.15252/embr.202255648

Figure 1. Generation of recombinant SARS–CoV‐2 harboring inactivating mutations in Nsp16.

Figure 1

  1. Schematic depiction of Nsp16 modifications within the bacterial artificial chromosome pBelo‐SARS–CoV‐2 Nsp16mut. Not drawn to scale.
  2. Inactivating mutations of aspartic acid residue 130 to Alanine (D130A) and lysine residue 170 to Alanine (K170A) within the active center of Nsp16, based on the publicly available crystal structure of Nsp16 (PDB 6W4H). SAM, S‐Adenosyl methionine.