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. 2022 Oct 24;23(12):e55175. doi: 10.15252/embr.202255175

Figure 4. PLAA and UBXN4 promote disassembly of ubiquitinated desmin filaments.

Figure 4

  1. shRNA‐mediated knockdown of Plaa and Ubxn4 in HEK293 cells. RT‐PCR of mRNA preparations from HEK293 cells expressing shLacz, shPlaa, or shUbxn4 using specific primers for PLAA or UBXN4. Data are plotted as the mean fold change relative to control ± SEM. n = 5 technical replicates (cells were plated in 5 wells per DNA plasmid). *P (shPlaa) z = 0.006, P (shUbxn4) = 0.015 vs. shLacz. Data are representative of one experiment.
  2. Downregulation of Plaa or Ubxn4 prevents the loss of desmin and soluble proteins in denervated muscle. Insoluble and soluble fractions from 14 days denervated muscles expressing shLacz, shPlaa, or shUbxn4 were analyzed by immunoblotting. n = 2 mice, biological replicates. Data are representative of one experiment.

Source data are available online for this figure.