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. 2022 Oct 24;23(12):e55044. doi: 10.15252/embr.202255044

Figure EV1. Verification of FBXW7 and APC knockout & genome‐wide screen quality control.

Figure EV1

  1. TIDE analysis of HPAF‐II FBXW7 −/− cell line and HPAF‐II APC −/− cell line.
  2. Immunoblot of lysates extracted from HPAF‐II FBXW7 −/− cell lines demonstrating knockout of FBXW7 protein expression.
  3. Fold‐change plots of HPAF‐II wild‐type and FBXW7 −/− genome‐wide screens demonstrating a change in essential genes at T24 of the screen.
  4. Precision‐recall curves of HPAF‐II wild‐type and FBXW7 −/− genome‐wide screens demonstrating training sets of essential and nonessential genes performed appropriately in the BAGEL algorithm.
  5. Immunoblot of lysates extracted from FBXW7 −/− cells following treatment with gRNAs targeting CCNL1.