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. 2022 Oct 24;23(12):e55044. doi: 10.15252/embr.202255044

Figure 2. Genome‐wide CRISPR screen performed in isogenic wild‐type and FBXW7 −/− HPAF‐II cells identifies a synthetic lethal genetic interaction.

Figure 2

  1. Schematic representation of genome‐wide CRISPR‐Cas9 dropout screens performed in isogenic wild‐type and FBXW7 −/− HPAF‐II cell lines.
  2. Differential Bayes Factor Z‐score plot comparing wild‐type and FBXW7 −/− genome‐wide dropout screens.
  3. Fold‐change abundance of individual gRNA targeting CCNL1 during the genome‐wide dropout screens from day 0 to day 24, n = 3 technical replicates per gRNA, mean ± SEM.
  4. Multicolour competition assay in both wild‐type and FBXW7 −/− cell lines, using mCherry‐AAVS1 and GFP‐GOI, normalized to AAVS1 control cells at each time‐point (n = 3 independent replicates), mean ± SEM, one‐way ANOVA.
  5. Proliferation assays in wild‐type and FBXW7 −/− cell lines show that knockout of CCNL1 preferentially affects FBXW7 −/− cells, normalized to AAVS1 control (n = 3 independent replicates), mean ± SEM, one‐way ANOVA.