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. 2022 Oct 24;23(12):e55044. doi: 10.15252/embr.202255044

Figure EV3. HPAF‐II FBXW7−/− and CCNL1OE cells exit mitosis faster than wild‐type.

Figure EV3

  1. Representative images of gating strategy for HPAF‐II wild‐type, FBXW7 −/− and CCNL1 OE cells to determine cell cycle distribution.
  2. Schematic of PIP‐FUCCI reporter marker expression through three major cell cycle phases.
  3. Live‐cell imaging of HPAF‐II wild‐type, FBXW7 −/− and CCNL1 OE cells expressing PIP‐FUCCI reporter treated with nocodazole overnight and released. Images collected over 3.5 h. Reduction in total population mCherry expression imaged over time, quantified in the Incucyte. Three independent replicates, mean ± SEM, two‐way ANOVA, *P = 0.0258, ****P < 0.0001.
  4. Live‐cell imaging of HPAF‐II wild‐type, FBXW7 −/− and CCNL1 OE cells expressing PIP‐FUCCI reporter either untreated or treated with nocodazole overnight and released. Measurement of individual cells as they lose mCherry expression. n = 15 cells per replicate, three independent replicates, one‐way ANOVA, mean ± SEM.