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. 2022 Oct 24;23(12):e55044. doi: 10.15252/embr.202255044

Figure 4. FBXW7 is involved in the ubiquitination of CCNL1.

Figure 4

  1. Immunoblot of immunoprecipitation of FLAG‐FBXW7 overexpressed in HEK293T cells, detecting endogenous CCNL1. Representative of three independent replicates.
  2. Immunoblot of immunoprecipitation of FLAG‐CCNL1 overexpressed in HEK293T cells, detecting endogenous FBXW7. Representative of three independent replicates.
  3. Immunoblot of cellular ubiquitination assay demonstrating the requirement of the 325‐TPALS‐329 degron for ubiquitination of CCNL1. Representative of three independent replicates.
  4. Immunoblot of cellular ubiquitination assay demonstrating the requirement of FBXW7 for ubiquitination of CCNL1. Representative of three independent replicates.
  5. Immunoblot of cellular ubiquitination assay showing re‐expression of FBXW7 in knockout cells leads to increased CCNL1 ubiquitination. Representative of three independent replicates.