Figure EV1. Disruptions in the epidermal stem cell compartments in aging skin.

• A
  Illustration of a mouse tail skin showing the slow‐ and fast‐cycling stem cells in the interfollicular epidermis (IFE) and their distinct differentiation program, giving rise to the interscale and scale regions, respectively.
• B, C
  The total number of boundary‐crossing clones in Dlx1^CreER and Slc1a3^CreER lineage‐tracing mice. Dlx1^CreER mice and chase time: N = 4 (12 months), N = 3 (16 months), and N = 9 (22 months). Slc1a3^CreER mice and chase time: N = 7 (12 months), N = 3 (16 months), and N = 8 (22 months). One‐way ANOVA, Dunn's multiple comparisons test. ns, not significant; *P < 0.05. Data show mean ± SD. N reflects biological replicates, which are summarized from at least two independent experiments.
• D, E
  Confocal imaging of representative clones at 2‐year‐chase, stained with K10 and K31. White boxes indicate areas that are enlarged in the lower panels. Z‐stack images show that the clones are originating from the basal layer and expanding into the upper differentiated layers. Cartoons summarize the sagittal view of the clones. Scale bar: 200 μm (upper panels), 20 μm (lower panels). K10 and K31 intensities were adjusted to similar levels between samples.
• F
  Classification of the border‐crossing clones from Dlx1^CreER and Slc1a3^CreER lineage‐tracing mice at 16‐month‐ and 22‐month‐chases. Images of these clones were represented in Fig 1I and EV1D and E.