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A
Slc1a3CreER lineage tracing in the Fbln7 WT and KO mice. Low‐dose tamoxifen was administered once at 2 months of age and samples were collected after 1‐week, 3‐month, and 1‐year chases. Wholemount staining of tail epidermis with tdTomato, K10, and Hoechst. Scale bar: 200 μm. WT, wild‐type; KO, knock‐out.
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B
The number of Slc1a3CreER+ clones per scale structure in 1‐week and 3‐month chases (left panel) and 1‐year chase (right panel).
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C
Quantitation of the area of Slc1a3CreER+ clones per total area of scale structure. Fbln7 WT mice for 1‐week (N = 5), 3‐month (N = 3), and 1‐year chases (N = 3). Fbln7 KO mice for 1‐week (N = 5), 3‐month (N = 4), and 1‐year chases (N = 6).
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D, E
Ki67 immunostaining in 1‐week‐chase mice (D) and its quantitation (E). White arrows show Ki67 and tdTomato double positive cells, which were counted and summarized in (E). Scale bar: 20 μm. N = 5 WT/het and 3 KO mice. Welch's t‐test.
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F
Representative pictures from tail wounds of 1‐year‐old Fbln7 WT and KO mice. Scale Bar: 4 mm.
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G
Measurements of wound area over time in Fbln7 WT (N = 5) and KO (N = 7) mice. Two‐way ANOVA, Tukey's multiple comparisons test.
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H–J
Hematoxylin and eosin staining from tail sections of wounded Fbln7 WT versus KO mice (H) and quantitation of the re‐epithelialization length (I) and thickness at the healing front (J). Scale bar: 200 μm. N = 3 WT and 5 KO mice.