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. 2022 Oct 10;23(12):e53065. doi: 10.15252/embr.202153065

Figure 2. Isolated autophagic vesicles are sealed.

Figure 2

  1. Differential interference contrast microscopy images of purified autophagic vesicles at high (I) or low (II) dilution. Images are representative of three independent approaches. Scale bar = 10 μm.
  2. Negative stain electron microscopy images of isolated vesicles. Scale bar = 500 nm.
  3. Size evaluation of isolated vesicles. The diameters of approx. 60 individual vesicles were determined using EM images. Statistics are depicted as mean ± SD.
  4. Western blot analysis of isolated autophagic vesicles upon proteinase K digestion. Mechanically opened vesicles served as positive control. For negative control, isolates were incubated with BSA instead of proteinase K. Depicted are two different blots that are representative for five independent experiments.