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. 2022 Oct 17;23(12):e54720. doi: 10.15252/embr.202254720

Figure 3. Characterization of the in vivo interactome of ROW in fly heads.

Figure 3

  1. Western blot for flies expressing endogenous FLAG‐tagged ROW (ROW‐FLAG), with αFlag tag antibody to validate the tagging. αTubulin (TUB) antibody was used as a reference. Het, Heterozygotes; Hom, Homozygotes for tagged ROW.
  2. Table summarizing the affinity purification–mass spectrometry data. IBAQ (intensity‐based absolute quantification) reflects the protein abundance in the sample. Peptide number is the number of razor and unique peptides. The data are the mean for the control (W1118, n = 3) and ROW‐FLAG flies (n = 3) samples. The proteins presented in the table were co‐purified with ROW in at least two out of the three experiments, none in the control experiments, and are supported by additional evidence from previous studies.
  3. Network of identified protein–protein interactions associated with ROW in fly heads. The lines represent previously identified interactions.
  4. BEAF‐32 coimmunoprecipitate with ROW. Lysates from S2 cells not transfected (−) or transfected (+) with ROW‐FLAG tagged plasmid were subjected to immunoprecipitation with αFlag tag beads. The immunoprecipitates and input (5%) were analyzed by western blot with αFlag tag and αBEAF‐32 antibodies.

Source data are available online for this figure.