Table 1.
Key resources.
| Reagent or Resource | Source | Identifier |
|---|---|---|
|
| ||
| Antibodies | ||
| Chicken anti-GFP (1:1000; 10 μg/mL) | Aves Labs | GFP 1020; RRID:AB_10000240 |
| Mouse anti-GFAP (1:1000) | Millipore Sigma | MAB360, RRID:AB_11212597 |
| Rabbit anti-SOX2 (1:500; 2 μg/mL) | Millipore Sigma | AB5603, RRID: AB_2286686 |
| Guinea pig anti-DCX (1:1000) | Millipore Sigma | AB2253, RRID: AB_1586992 |
| Rat anti-BrdU (1:500; 2 μg/mL) | BioRad | MCA2060GA,RRID: AB_10545551 |
| Goat anti-chicken IgY Alexa Fluor 488 (1:1000; 2 μg/mL) | Molecular Probes | A-11039, RRID: AB_142924 |
| Goat anti-rabbit IgG Alexa Fluor 568 (1:1000; 2 μg/mL) | Molecular Probes | A-11036, RRID:AB_10563566 |
| Goat anti-rat IgG Alexa Fluor 568 (1:1000; 2 μg/mL) | Molecular Probes | A-11077, RRID: AB_141874 |
| Goat anti-mouse IgG Alexa Fluor 647 (1:1000; 2 μg/mL) | Thermo Fisher Scientific | A-21242, RRID: AB_2535811 |
| goat anti-guinea pig IgG Alexa Fluor 647 (1:1000; 2 μg/mL) | Thermo Fisher Scientific | A-21450, RRID: AB_2735091 |
| DAPI (1:2000) | ThermoFisher Scientific | 62248 |
| Prolong Diamond with DAPI | ThermoFisher Scientific | P36966 |
| Chemicals and Instruments | ||
| L-15 Medium | Gibco | 11415–064 |
| Fibroblast growth factor | Sigma | F0291 |
| Epidermal growth factor | Invitrogen | 53303–-018 |
| Caspase Inhibitor | Promega | G7231 |
| B27 | Gibco | 17504-–044 |
| HBSS powder | Sigma | H4891 |
| 2.5% Trypsin (10×) | Gibco | 15090–046 |
| Trypsin Inhibitor | Gibco | 007–100 |
| Scopolamine methyl bromide | Sigma | S8502–1G |
| Pilocarpine | Sigma | P6503–5G |
| Paraformaldehyde | Electron Microscopy Sciences | 15174–S |
| Heparin Sodium | Fisher BioReagents | BP245 1 g |
| Midazolam HCL | Covetrus | 23155060142 |
| Fatal Plus | Covetrus | VPL9373 |
| Isothesia | Henry Schein | SKU 029405 |
| Glass syringe (5 μl, removable needle) | Hamilton | 7634–01 |
| Needle 26GA, 0.5″ 30° | Hamilton | 7804–03 |
| Automated stereotaxic injection system | Stoelting Quintessential | 53311 |
| Charcoal filter, VetEquip | Harvard Apparatus | 340415 |
| Stereotaxic Apparatus | KOPF | 940 Small |
| Experimental Model: Strains | ||
| C57BL/6NHsd | Envigo | 044 |
| B6.Cg-Tg(Slc32a1-COP4*H134R/EYFP)8Gfng/J (VGAT-ChR2-EYFP line 8) | The Jackson Laboratory | 014548 |
| Software | ||
| Graphpad Prism 7 | ||