Table 2.
Statistical comparison.
| Fig. 1: Type 1 progenitors | ||||
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| Quantitative measurements | Naïve-Media vs. Naïve-TX | Naïve-Media vs. TLE- Media | Naïve-Media vs. TLE- TX | TLE – Media vs. TLE- TX |
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| ANOVA: p value | 0.31 | 0.002 | 0.0002 | 0.31 |
| Mean (cells) | Naïve-Media: 235 | Naive-Media: 235 | Naive-Media: 235 | TLE - Media: 138 |
| Naïve-TX: 265 | TLE- Media: 138 | TLE- TX: 107 | TLE- TX: 107 | |
| Difference | −30 | 97 | 128 | 31 |
| N (animals) | Naive- Media: 4 | Naive- Media: 4 | Naive- Media: 4 | TLE - Media: 4 |
| Naive-TX: 4 | TLE- Media: 4 | TLE- TX: 6 | TLE- TX: 6 | |
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| Fig. 1: Inverted Type 1 progenitors | ||||
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| Quantitative measurements | Naïve-Media vs. Naïve-TX | Naïve-Media vs. TLE- Media | Naïve-Media vs. TLE- TX | TLE- Media vs. TLE- TX |
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| ANOVA: p value | 0.99 | <0.0001 | 0.002 | <0.0001 |
| Mean (cells) | Naïve-Media: 14 | Naive-Media: 14 | Naive-Media: 14 | TLE- Media: 156 |
| Naïve-TX: 18 | TLE- Media: 156 | TLE- TX: 70 | TLE- TX: 70 | |
| Difference | −4 | −142 | −56 | 86 |
| N (animals) | Naive- Media: 4 | Naive- Media: 4 | Naive- Media: 4 | TLE- Media: 4 |
| Naive-TX: 4 | TLE- Media: 4 | TLE- TX: 6 | TLE- TX: 6 | |
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| Fig. 1: Type 2 progenitors | ||||
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| Quantitative measurements | Naïve-Media vs. Naïve-TX | Naïve-Media vs. TLE- Media | Naïve-Media vs. TLE - TX | TLE- Media vs. TLE- TX |
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| ANOVA: p value | 0.04 | 0.0008 | 0.01 | <0.0001 |
| Mean (cells) | Naïve-Media: 1256 | Naive-Media: 1256 | Naive-Media: 1256 | TLE- Media: 1926 |
| Naïve-TX: 871 | TLE- Media: 1926 | TLE- TX: 851 | TLE- TX: 851 | |
| Difference | 385 | −670 | 405 | 1075 |
| N (animals) | Naive- Media= 4 | Naive- Media= 4 | Naive- Media= 4 | TLE- Media= 4 |
| Naïve-TX= 4 | TLE- Media= 4 | TLE- TX= 6 | TLE- TX= 6 | |
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| Fig. 2: DCX+ cells in GCL | ||||
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| Quantitative measurements | Naïve-Media vs. Naïve- TX | Naïve-Media vs. TLE- Media | Naïve-Media vs. TLE- TX | TLE- Media vs. TLE- TX |
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| ANOVA: p value | 0.02 | <0.0001 | <0.0001 | 0.0003 |
| Mean (cells) | Naïve-Media: 2299 | Naive-Media: 2299 | Naive-Media: 2299 | TLE- Media: 3667 |
| Naïve- TX: 2840 | TLE- Media: 3667 | TLE- TX: 4516 | TLE- TX: 4516 | |
| Difference | −541 | −1368 | −2217 | −-849 |
| N (animals) | Naïve-Media: 5 | Naive- Media: | Naive- Media: | TLE- Media: |
| Naïve- TX: 4 | TLE- Media: | TLE- TX: | TLE- TX: | |
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| Fig. 2: DCX+ cells in Hilus | ||||
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| Quantitative measurements | Naïve-Media vs. Naïve- TX | Naïve-Media vs. TLE- Media | Naïve-Media vs. TLE- TX | TLE- Media vs. TLE- TX |
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| ANOVA: p value | 0.8 | <0.0001 | <0.0001 | 0.0009 |
| Mean (cells) | Naïve-Media: 22 | Naive-Media: 22 | Naive-Media: 22 | TLE- Media: 550 |
| Naïve- TX: 30 | TLE- Media: 550 | TLE- TX: 335 | TLE- TX: 335 | |
| Difference | −8 | −528 | −313 | 215 |
| N (animals) | Naïve-Media: 5 | Naive- Media: 5 | Naive- Media: 5 | TLE- Media: 5 |
| Naïve- TX:4 | TLE- Media: 5 | TLE- TX: 6 | TLE - TX: 6 | |
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| Fig. 2: Correlation between TX and DCX+ cells | ||||
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| Quantitative measurements | Naïve- TX | TLE- TX | ||
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| Pearson correlation: R | 0.95 | 0.83 | ||
| P value | 0.04 | 0.03 | ||
| R squared | 0.91 | 0.695 | ||
| N (animals) | Naïve- TX: 4 | TLE- TX: 6 | ||
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| Fig. 2: Migration distance analysis | ||||
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| Quantitative measurements | Naïve-Media vs. Naïve- TX | Naïve-Media vs. TLE-Media | Naïve-Media vs. TLE-TX | TLE- Media vs. TLE- TX |
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| Kolmogorov- Smirnov test: p value | 0.03 | 0.01 | 0.009 | 0.3 |
| Mean (μm) | Naïve-Media: 9.06 | Naive-Media: 9.06 | Naive-Media: 9.06 | TLE- Media: 16.37 |
| Naïve- TX: 16.86 | TLE- Media: 16.37 | TLE- TX: 24.5 | TLE- TX: 24.5 | |
| Difference | −7.8 | −7.31 | −15.44 | −8.13 |
| N (cells) | Naive-Media: 38 | Naive- Media: 38 | Naive- Media: 38 | TLE- Media: 90 |
| Naïve- TX: 91 | TLE- Media: 90 | TLE- TX: 141 | TLE- TX: 141 | |
| N (Animals) | Naïve-Media: 5 | Naive- Media: 5 | Naive- Media: 5 | TLE- Media: 5 |
| Naïve- TX: 4 | TLE- Media: 5 | TLE- TX: 6 | TLE - TX: 6 | |
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| Fig. 3: Distribution of DCX+ cells (GCL), Distance from Bregma: −2.04 mm | ||||
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| Quantitative measurements | Naïve-Media vs. Naïve- TX | Naive-Media vs. TLE- Media | Naïve-Media vs. TLE- TX | TLE- Media vs. TLE- TX |
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| ANOVA: p value | 0.03 | 0.34 | 0.0003 | 0.009 |
| Mean (cells) | Naïve-Media: 1828 | Naive-Media: 1828 | Naive-Media: 1828 | TLE- Media: 2048 |
| Naïve- TX: 2251 | TLE- Media: 2048 | TLE- TX: 2497 | TLE- TX: 2497 | |
| Difference | −422.6 | −220 | −669 | −449 |
| N (animals) | Naïve-Media: 5 | Naive-Media: 5 | Naive-Media: 5 | TLE- Media: 5 |
| Naïve- TX: 4 | TLE- Media: 5 | TLE- TX: 6 | TLE- TX: 6 | |
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| Fig. 3: Distribution of DCX+ cells (GCL), Distance from Bregma: −2.36 mm | ||||
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| Quantitative measurements | Naïve-Media vs. Naïve- TX | Naïve-Media vs. TLE- Media | Naïve-Media vs. TLE- TX | TLE- Media vs. TLE- TX |
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| ANOVA: p value | 0.57 | <0.0001 | <0.0001 | <0.0001 |
| Mean (cells) | Naïve-Media: 208 | Naive-Media: 208 | Naive-Media: 208 | TLE- Media: 736 |
| Naïve- TX: 274 | TLE- Media: 736 | TLE- TX: 834 | TLE- TX: 1042 | |
| Difference | −66 | −528 | −834 | −306 |
| N (animals) | Naïve-Media: 5 | Naive-Media: 5 | Naive-Media: 5 | TLE- Media: 5 |
| Naïve- TX: 4 | TLE- Media: 5 | TLE- TX: 6 | TLE- TX: 6 | |
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| Fig. 3: Distribution of DCX+ cells (GCL), Distance from Bregma: −2.96 mm | ||||
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| Quantitative measurements | Naïve-Media vs. Naïve- TX | Naïve-Media vs. TLE- Media | Naïve-Media vs. TLE- TX | TLE- Media vs. TLE- TX |
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| ANOVA: P value | 0.99 | 0.001 | 0.0002 | 0.87 |
| Mean (cells) | Naïve-Media: 149 | Naive-Media: 149 | Naive-Media: 149 | TLE- Media: 490 |
| Naïve- TX: 162 | TLE- Media: 490 | TLE- TX: 542 | TLE- TX: 542 | |
| Difference | −13 | −341 | −393 | −52 |
| N (animals) | Naïve-Media: 5 | Naive-Media: 5 | Naive-Media: 5 | TLE- Media: 5 |
| Naïve- TX: 4 | TLE- Media: 5 | TLE- TX: 6 | TLE- TX: 6 | |
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| Fig. 3: Distribution of DCX+ cells (GCL), Distance from Bregma: −3.28 mm | ||||
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| Quantitative measurements | Naïve-Media vs. Naïve- TX | Naïve-Media vs. TLE- Media | Naïve-Media vs. TLE- TX | TLE- Media vs. TLE- TX |
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| ANOVA: p value | 0.99 | 0.001 | 0.0002 | 0.87 |
| Mean (cells) | Naïve-Media: 113 | Naive-Media: 113 | Naive-Media: 113 | TLE- Media: 392 |
| Naïve- TX: 152 | TLE- Media: 392 | TLE- TX: 417 | TLE- TX: 417 | |
| Difference | −39 | −279 | −304 | −25 |
| N (animals) | Naïve-Media: 5 | Naive-Media: 5 | Naive-Media: 5 | TLE- Media: 5 |
| Naïve- TX: 4 | TLE- Media: 5 | TLE- TX: 6 | TLE- TX: 6 | |
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| Fig. 3: Distribution of DCX+ cells (Hilus) TLE-Media vs. TLE-TX | ||||
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| Quantitative measurements | −2.04 mm | −2.36 mm | −2.96 mm | −3.28 mm |
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| Multiple t-test: p value | 0.02 | 0.008 | 0.54 | 0.86 |
| Mean (cells) | TLE-Media: 335 | TLE-Media: 114 | TLE-Media: 68 | TLE- Media: 52 |
| TLE- TX: 114 | TLE- TX: 57 | TLE- TX: 47 | TLE- TX: 55 | |
| Difference | 221 | 57 | 21 | −3 |
| N (animals) | TLE-Media: 5 | TLE-Media: 5 | TLE-Media: 5 | TLE-Media: 5 |
| TLE-TX: 6 | TLE-TX: 6 | TLE-TX: 6 | TLE-TX: 6 | |
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| Fig. 4: Cell proliferation (BrdU 2-h) | ||||
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| Quantitative measurements | Naïve-Media vs. Naïve- TX | Naïve-Media vs. TLE- Media | Naïve-Media vs. TLE- TX | TLE- Media vs. TLE- TX |
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| ANOVA: p value | 0.86 | <0.0001 | <0.0001 | >0.999 |
| Mean (cells) | Naïve-Media: 285 | Naive-Media: 285 | Naive-Media: 285 | TLE- Media: 478 |
| Naïve- TX:300 | TLE- Media: 478 | TLE- TX: 479 | TLE- TX: 479 | |
| Difference | −15 | −193 | −194 | −1 |
| N (animals) | Naïve-Media: 5 | Naive- Media 5 | Naive- Media: 5 | TLE- Media: 4 |
| Naïve- TX: 4 | TLE- Media: 4 | TLE- TX: 4 | TLE- TX: 4 | |
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| Fig. 5: Survival (BrdU 3-weeks) | ||||
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| Quantitative measurements | Naïve-Media vs. Naïve- TX | Naïve-Media vs. TLE- Media | Naïve-Media vs. TLE- TX | TLE- Media vs. TLE- TX |
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| Kruskal-Wallis: p value | 0.04 | 0.051 | 0.0001 | 0.04 |
| Mean (rank) | Naïve-Media: 3 | Naive-Media: 3 | Naive-Media: 3 | TLE- Media: 9.8 |
| Naïve- TX: 10.5 | TLE- Media: 9.8 | TLE- TX: 16.8 | TLE- TX: 16.8 | |
| Difference | −7.5 | −6.8 | −13.8 | −7 |
| N (animals) | Naïve-Media: 5 | Naive- Media: 5 | Naive- Media: 5 | TLE- Media: 5 |
| Naïve- TX: 4 | TLE- Media: 5 | TLE- TX: 5 | TLE- TX: 5 | |
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| Fig. 6: Distribution of BrdU+ cells (GCL), Distance from Bregma: −2.04 mm | ||||
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| Quantitative measurements | Naïve-Media vs. Naïve- TX | Naïve-Media vs. TLE- Media | Naïve-Media vs. TLE- TX | TLE- Media vs. TLE- TX |
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| Kruskal-Wallis: P value | 0.01 | 0.16 | <0.0001 | 0.01 |
| Mean (rank) | Naïve-Media: 3 | Naive-Media: 3 | Naive-Media: 3 | TLE- Media: 8 |
| Naïve- TX: 12.5 | TLE- Media: 8 | TLE- TX: 17 | TLE- TX: 17 | |
| Difference | −13 | −5 | −14 | −9 |
| N (animals) | Naïve-Media: 5 | Naive-Media: 5 | Naive-Media: 5 | TLE- Media: 5 |
| Naïve- TX: 4 | TLE- Media: 5 | TLE- TX: 5 | TLE- TX: 5 | |
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| Fig. 6: Distribution of BrdU+ cells (GCL), Distance from Bregma: −2.36 mm | ||||
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| Quantitative measurements | Naïve-Media vs. Naïve- TX | Naïve-Media vs. TLE- Media | Naïve-Media vs. TLE- TX | TLE- Media vs. TLE- TX |
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| Kruskal-Wallis: P value | 0.01 | 0.16 | 0.0002 | 0.051 |
| Mean (rank) | Naïve-Media: 3 | Naive-Media: 3 | Naive-Media: 3 | TLE- Media: 9.3 |
| Naïve- TX: 11.8 | TLE- Media: 9.3 | TLE- TX: 16.2 | TLE- TX: 16.2 | |
| Difference | −8.8 | −6.3 | −13.2 | −6.9 |
| N (animals) | Naïve-Media: 5 | Naive-Media: 5 | Naive-Media: 5 | TLE- Media: 5 |
| Naïve- TX: 4 | TLE- Media: 5 | TLE- TX: 5 | TLE- TX: 5 | |
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| Fig. 6: Distribution of BrdU+ cells (GCL), Distance from Bregma: −2.96 mm | ||||
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| Quantitative measurements | Naïve-Media vs. Naïve- TX | Naïve-Media vs. TLE- Media | Naïve-Media vs. TLE- TX | TLE- Media vs. TLE- TX |
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| Kruskal-Wallis: P value | 0.055 | 0.01 | 0.13 | 0.33 |
| Mean (rank) | Naïve-Media: 4.8 | Naive-Media: 4.8 | Naive-Media: 4.8 | TLE- Media: 13.5 |
| Naïve- TX: 12 | TLE- Media: 13.5 | TLE- TX: 10.1 | TLE- TX: 10.1 | |
| Difference | −7.2 | −8.7 | −5.3 | 3.4 |
| N (animals) | Naïve-Media: 5 | Naive-Media: 5 | Naive-Media: 5 | TLE- Media: 5 |
| Naïve- TX: 4 | TLE- Media: 5 | TLE- TX: 5 | TLE- TX: 5 | |
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| Fig. 6: Distribution of BrdU+ cells (GCL), Distance from Bregma: −3.28 mm | ||||
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| Quantitative measurements | Naïve-Media vs. Naïve- TX | Naïve-Media vs. TLE- Media | Naïve-Media vs. TLE- TX | TLE- Media vs. TLE- TX |
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| Kruskal-Wallis: p value | 0.053 | 0.003 | 0.006 | 0.91 |
| Mean (rank) | Naïve-Media: 3.2 | Naive-Media: 3.2 | Naive-Media: 3.2 | TLE- Media: 13.8 |
| Naïve- TX: 12 | TLE- Media: 13.5 | TLE- TX: 10.1 | TLE- TX: 13.4 | |
| Difference | −7.2 | −10.6 | −10.2 | 0.4 |
| N (animals) | Naïve-Media: 5 | Naive-Media: 5 | Naive-Media: 5 | TLE- Media: 5 |
| Naïve- TX: 4 | TLE- Media: 5 | TLE- TX: 5 | TLE- TX: 5 | |
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| Supplementary Fig. 1: Effect of midazolam on cell proliferation | ||||
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| Quantitative measurements | Naïve-Saline vs. Naïve- Midazolam | Naïve-Saline vs. TLE- Midazolam | Naïve-Midazolam vs. TLE- Midazolam | |
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| ANOVA: p value | 0.997 | 0.0003 | 0.006 | |
| Mean (cells) | Naïve-Saline: 235 | Naive-Saline: 235 | Naive-Midazolam: 236 | |
| Naïve- Midazolam: 236 | TLE- Midazolam: 578 | TLE- Midazolam: 578 | ||
| Difference | −1 | −343 | −342 | |
| N (animals) | Naïve-Saline: 3 | Naive-Saline: 3 | Naive-Saline: 3 | |
| Naïve- Midazolam: 3 | TLE- Midazolam: 3 | TLE- Midazolam: 3 | ||