Figure 3.
HPCA1 is not required for systemic cell-to-cell changes in membrane potential in response to light stress. Arabidopsis plants were subjected to a HL) stress treatment applied to a single leaf (Local; indicated with a circle), and changes in membrane potential were imaged using DiBAC4(3) in whole plants (local and systemic tissues). Representative time-lapse images of whole-plant changes in membrane potential in WT, hpca1-1 and hpca1-2 plants are shown alongside bar graphs of combined data from all plants used for the analysis at the 0- and 30-min time points (local and systemic). The double mutant glr3.3 glr3.6, that lacks a cell-to-cell membrane potential signal in response to HL stress (Fichman and Mittler 2021a), was used as a negative control. All experiments were repeated at least three times with 10 plants of each genotype per experiment. Data are presented as box plot graphs; X is mean ± se, N = 30, **P < 0.01, Student t-test. Scale bar, 1 cm. See Movie 1 for live imaging.