Western immunoblot analysis demonstrating reactivity to P. carinii antigens. IgG in serum (A), IgA in serum (B), IgG in BAL (C), and IgA in BAL (D) are shown. Lane 1, HIV-positive patient with Pcp; lane 2, HIV-positive patient without Pcp; lane 3, HIV-negative control subject. The same three patients were used in panels A to D.