FIG. 2.

Cytotoxic effects of bacterial supernatant (S. chromogenes 690LR) on Int 407 cells. B-broth and bacterial cultural supernatants were diluted in BEM, loaded onto a Int 407 cell monolayer, and incubated for 20 h at 37°C in 95% air and 5% CO₂. (A) B-broth at a 1:2.5 dilution in BEM caused no cytopathic effects on cultured Int 407 cells. (B) Bacterial supernatants at a 1:2.5 dilution in BEM caused rounding and clumping of Int 407 cells.