FIG. 3.

Cytotoxic effects of bacterial supernatant (S. chromogenes 690LR) on CHO-K1 cells. B-broth and bacterial cultural supernatants were diluted in Ham F-12 medium, loaded onto an Int 407 cell monolayer, and incubated for 20 h at 37°C in 95% air and 5% CO₂. (A) B-broth at a 1:2.5 dilution in Ham F-12 medium caused no cytopathic effects on the cultured CHO-K1 cells. (B) Bacterial supernatants at 1:2.5 and 1:5 dilutions in Ham F-12 medium caused the rounding of CHO-K1 cells.