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. 2000 Mar;68(3):1102–1108. doi: 10.1128/iai.68.3.1102-1108.2000

FIG. 6.

FIG. 6

The 34- to 36-kDa protein was heat labile. The same amounts of concentrated supernatants were used for loading treated and untreated wells. For heat treatment, the supernatants were heated at 65°C for 30 min. (A) SDS-PAGE. MW, low-range protein molecular mass marker (in kilodaltons) (Bio-Rad). Lanes 1, 3, 5, and 7, untreated supernatants from S. chromogenes strains 1LR, 690LR, 302RR, and 726LR; lanes 2, 4, 6, and 8, the corresponding heat-treated supernatants. (B) Western blots. MW, low-range protein molecular mass marker (in kilodaltons) (Bio-Rad). Lanes 1, 3, 5, and 7, untreated supernatants from S. chromogenes strains 1LR, 690LR, 302RR, and 726LR; lanes 2, 4, 6, and 8, the corresponding heat-treated supernatants. The polyclonal antibody was raised in rabbits against the 34- to 36-kDa protein purified from S. chromogenes 690LR.