FIG. 9.

Casein hydrolysis activity of the 34- to 36-kDa protein from S. chromogenes 302RR. The white spot represents hydrolysis of casein. The crude supernatant was separated by nondenatured PAGE. After 10 min of washing in Tris-HCl buffer with 2 mM CaCl₂, the gel was laid over a casein plate. The result was recorded after 2 h of incubation at 37°C.