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. 2000 Mar;68(3):1102–1108. doi: 10.1128/iai.68.3.1102-1108.2000

TABLE 1.

Cytotoxic assaya

Species Total no. No. of isolates (% of total no.) showing:
Cell lysis Cell rounding
S. aureus 8 6 (75) 0 (0)
S. chromogenes 26 0 (0) 24 (92.3)
S. hyicus 7 0 (0) 1 (14.3)
Other CoNS sp. 16 3 (18.8) 1 (6.3)
a

Bacteria were grown in B-broth overnight; supernatants were then diluted 1:2.5 and 1:5 in MEM and loaded onto HEp-2 cell monolayers. After 20 h of incubation at 37°C in 95% air and 5% CO2, the cells were examined microscopically for morphological changes. Cell lysis was indicated by HEp-2 cell destruction. Cell rounding indicated HEp-2 cells detached from the plastic cultural surface and floating in the cell culture medium.