TABLE 1.
Cytotoxic assaya
Species | Total no. | No. of isolates (% of total no.) showing:
|
|
---|---|---|---|
Cell lysis | Cell rounding | ||
S. aureus | 8 | 6 (75) | 0 (0) |
S. chromogenes | 26 | 0 (0) | 24 (92.3) |
S. hyicus | 7 | 0 (0) | 1 (14.3) |
Other CoNS sp. | 16 | 3 (18.8) | 1 (6.3) |
Bacteria were grown in B-broth overnight; supernatants were then diluted 1:2.5 and 1:5 in MEM and loaded onto HEp-2 cell monolayers. After 20 h of incubation at 37°C in 95% air and 5% CO2, the cells were examined microscopically for morphological changes. Cell lysis was indicated by HEp-2 cell destruction. Cell rounding indicated HEp-2 cells detached from the plastic cultural surface and floating in the cell culture medium.