TABLE 2.

Inhibition of the 34- to 36-kDa protein activity by various inhibitors^a

Inhibitor	Type	Concn	Remaining activity (%)
EDTA	Metal chelator	5 mM	0
1,10-phenanthroline	Metal chelator	1–2 mM	0
Chelax-100	Metal chelator	1 g/10 ml	0
PMSF	Serine protease inhibitor	1–2 mM	100

^aThe purified 34- to 36-kDa protease was treated with different inhibitors for 30 min at room temperature; the protease activity was then measured at 37°C for 45 min as described in Materials and Methods. The untreated protease was used as the standard for 100% activity.