FIG. 3.

Enzyme-linked immunosorbent assay quantitation of histone-associated DNA fragments in the cytoplasm of human neutrophils incubated in vitro with freshly prepared host cell-free or purified HGE agent. Neutrophils incubated with freshly freed or purified HGE agent had significantly (∗, P < 0.05; ∗∗, P < 0.01) increased cytoplasmic histone-associated DNA fragments compared with neutrophils incubated with medium alone (n = 3 assays using neutrophils derived from a single donor). A representative experiment is shown from three independent experiments performed, each with neutrophils derived from different donors and freshly prepared host cell-free HGE agent.