Table 2. Summary of cellular species, culture conditions, and observation setup used in the experiments in Figure 5.

Species	Label	Strain	Medium	Temperature (°C)	Device
E. coli	rpoS-mcherry glucose_30°C	MG1655 F3 rpoS-mcherry /pUA66-PrpsL-gfp	M9 minimal medium +0.2%(w/v) glucose +1/2 MEM amino acids solution (Sigma)	30	Microchamber array	This study
E. coli	rpoS-mcherry glucose_37°C	MG1655 F3 rpoS-mcherry /pUA66-PrpsL-gfp	M9 minimal medium +0.2%(w/v) glucose +1/2 MEM amino acids solution (Sigma)	37	Microchamber array	This study
E. coli	rpoS-mcherry glycerol_37°C	MG1655 F3 rpoS-mcherry /pUA66-PrpsL-gfp	M9 minimal medium +0.1%(v/v) glycerol +1/2 MEM amino acids solution (Sigma)	37	Microchamber array	This study
E. coli	f3nw -sm	F3NW	M9 minimal medium +0.2%(w/v) glucose +1/2 MEM amino acids solution (Sigma)+0.1mM Isopropyl β-D-1 thiogalactopyranoside (IPTG)	37	Agar pad	Nozoe et al., 2017
E. coli	f3nw +sm	F3NW	M9 minimal medium +0.2%(w/v) glucose +1/2 MEM amino acids solution (Sigma)+0.1 mM Isopropylβ-D-1 thiogalactopyranoside (IPTG)+100 μg/ml streptomycin	37	Agar pad	Nozoe et al., 2017
E. coli	f3ptn001 -sm	F3/pTN001	M9 minimal medium +0.2%(w/v) glucose +1/2 MEM amino acids solution (Sigma)+0.1 mM Isopropylβ-D-1 thiogalactopyranoside (IPTG)	37	Agar pad	Nozoe et al., 2017
E. coli	f3ptn001+sm	F3/pTN001	M9 minimal medium +0.2%(w/v) glucose +1/2 MEM amino acids solution (Sigma)+0.1 mM Isopropylβ-D-1 thiogalactopyranoside (IPTG)+200 μg/ml streptomycin	37	Agar pad	Nozoe et al., 2017
M. smegmatis	mc2155 7H9	mc2155	Middlebrook 7H9 medium +0.5% albumin +0.2% glucose +0.085% NaCl+0.5% glycerol +0.05% Tween-80	37	Membrane cover	Wakamoto et al., 2013
S. pombe	EMM28	HN0025	Edinburgh minimal medium +2% (w/v) glucose	28	Mother machine	Nakaoka and Wakamoto, 2017
S. pombe	EMM30	HN0025	Edinburgh minimal medium +2%(w/v) glucose	30	Mother machine	Nakaoka and Wakamoto, 2017
S. pombe	EMM32	HN0025	Edinburgh minimal medium +2%(w/v) glucose	32	Mother machine	Nakaoka and Wakamoto, 2017
S. pombe	EMM34	HN0025	Edinburgh minimal medium +2%(w/v) glucose	34	Mother machine	Nakaoka and Wakamoto, 2017
S. pombe	YE28	HN0025	Yeast extract medium +3%(w/v) glucose	28	Mother machine	Nakaoka and Wakamoto, 2017
S. pombe	YE30	HN0025	Yeast extract medium +3%(w/v) glucose	30	Mother machine	Nakaoka and Wakamoto, 2017
S. pombe	YE34	HN0025	Yeast extract medium +3%(w/v) glucose	34	Mother machine	Nakaoka and Wakamoto, 2017
L1210 mouse leukemia cell	L1210 RPMI-1640	L1210 (ATCC CCL-219)	RPMI-1640 medium (Wako)+10% fetal bovine serum (Biosera) under 5% CO2 atmosphere	37	Mother machine	Seita et al., 2021