FIG. 1.

Medium conditioned by endothelial cells plus C. albicans stimulates uninfected endothelial cells to secrete IL-8. Endothelial cells (EC) were incubated with media conditioned by the indicated stimuli for 8 h. The net concentration of IL-8 was calculated by subtracting the concentration of IL-8 in the conditioned medium before it was added to the endothelial cells from the concentration of IL-8 in the medium after it had been exposed to the endothelial cells for 8 h. The IL-8 concentrations were measured by ELISA. Results are mean ± standard deviations of three separate experiments. ∗, P ≤ 0.01 compared to medium conditioned by endothelial cells alone.