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Effects of Stellettin B on the MAPKs signaling pathway. HA22T and HepG2 cells were treated with different concentrations of Stellettin B (0, 6, 12, 18, and 24 μg/mL) and cell lysates were collected for western blotting analysis. The MAPKs-related proteins were validated, including p38MAPK, p-p38MAPK, ERK, p-ERK, JNK, p-JNK, JUN, and p-JUN. Zero refers to cells treated with DMSO vehicle as a control. β-Actin was used as the internal control.
