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. 2022 May 19;20(6):465–474. doi: 10.2450/2022.0013-22

Figure 1. GIFT and GAT phenotyping.

Figure 1

(A) CTL-2 protein expression level was evaluated on surface of freshly isolated neutrophils. Easy-Sep isolated neutrophils were incubated with rabbit polyclonal anti-human CTL-2 antibody (0.2 mg/mL) for 60 min. After 2 washes, cells were incubated with an anti-Rabbit 488-conjugated antibody for 30 min. Following incubation, samples were analysed on FACSCanto and MFI was recorded. Total leukocytes were isolated from 56 healthy volunteers and GIFT (B) and GAT (C) were performed with one anti-HNA-3 serum (anti-HNA-3a serum #1). GIFT (D) and GAT (E) were repeated on Easy-Sep isolated neutrophils from 19 and 15 healthy volunteers, respectively, using a second anti-HNA-3a serum (anti-HNA-3a serum #2). CTL-2 and GIFT against anti-HNA serum #2 data are presented as mean ± SE. GIFT against anti-HNA-3 serum #1 and GAT data are presented as median and IQR. P <0.05 was considered statistically significant.