Table IV.

HLA/HNA antibody investigations in TRALI cases between 1999–2019

Investigations		All	Antibody mediated TRALI	Non-antibody mediated TRALI	Antibody mediated possible TRALI	Non-antibody mediated possible TRALI	Uncategorised TRALIa
Number of cases [cases (% of all cases)]		48	11 (22.9%)	10 (20.8%)	12 (25.0%)	6 (12.5%)	9 (18.8%)
HLA/HNA antibody screen [cases (% of cases for column)]	Antibodies detected	36 (75.0%)	11 (100%)	1 (10.0%)	12 (100%)	3 (50.0%)	9 (100%)
	• Concordant	23 (63.9%)	11 (100%)	0 (0%)	12 (100%)	0 (0%)	N/A
	• Non-concordant	4 (11.1%)	0 (0%)	1 (10.0%)	0 (0%)	3 (50.0%)	N/A
	No antibodies detected	12 (25.0%)	0 (0%)	9 (90.0%)	0 (0%)	3 (50.0%)	0 (0%)
Concordant antibody specificity [cases (% of cases for column)]	HLA Class I only	4 (8.3%)	1 (9.1%)	0 (0%)	3 (27.3%)	0 (0%)	N/A
	HLA Class II only	0 (0%)	0 (0%)	0 (0%)	0 (0%)	0 (0%)	N/A
	HNA only	1 (2.1%)	1 (9.1%)	0 (0%)	0 (0%)	0 (0%)	N/A
	Multiple specificitiesb	18 (37.5%)	9 (81.2%)	0 (0%)	9 (75.0%)	0 (0%)	N/A
	HLA class I +II	4 (8.3%)	2 (18.2%)	0 (0%)	2 (16.7%)	0 (0%)	N/A
	HLA class I + HNA	3 (6.3%)	0 (0%)	0 (0%)	3 (25.0%)	0 (0%)	N/A
	HLA class II + HNA	4 (8.3%)	3 (27.2%)	0 (0%)	1 (8.3%	0 (0%)	N/A
	HLA class I/II + HNA	7 (14.6%)	4 (36.4%)	0 (0%)	3 (25.0%)	0 (0%)	N/A
Non-concordant antibody specificity [cases (% of cases for column)]	HLA Class I only	3 (6.3%)	0 (0%)	1 (10.0%)	0 (0%)	2 (33.3%)	N/A
	HLA Class II only	0 (0%)	0 (0%)	0 (0%)	0 (0%)	0 (0%)	N/A
	HNA only	0 (0%)	0 (0%)	0 (0%)	0 (0%)	0 (0%)	N/A
	Multiple specificities	1 (2.1%)	0 (0%)	0 (0%)	0 (0%)	1 (16.7%)	N/A
	HLA class + II	1 (2.1%)	0 (0%)	0 (0%)	0 (0%)	1 (16.7%)	N/A

^aUncategorised TRALI cases lacked donor testing to define concordance, and in these cases antibody detection was as follows: only HLA class I in 1 (11.1%) case, only HLA class II antibodies in 3 (33.3%) cases, only HNA antibodies in 2 (22.2%) cases and multiple specificities in 3 (33.3%) cases.

^bSome cases had more than one antibody type.