REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-human CD3 PerCP clone SK7	BD Biosciences	Cat#345766; RRID:AB_2783791
Anti-human CD4 V450 clone L200	BD Biosciences	Cat#560811; RRID:AB_2033927
Anti-human CD8 FITC clone DK25	Agilent	Cat#F076501-2; RRID:AB_578668
Anti-human CCR7 BV711 clone 150503	BD Biosciences	Cat#566602; RRID:AB_2739758
Anti-human CD45RA PECy7 clone L48	BD Biosciences	Cat#337186; RRID:AB_2828012
Anti-human CD69 APC-H7 clone FN50	BD Biosciences	Cat#560737; RRID:AB_1727508
Anti-human CD137 PE clone 4B4-1	Miltenyi Biotec	Cat#130-119-885; RRID:AB_2783944
Anti-human OX40 BV605 clone L106	BD Biosciences	Cat#745217; RRID:AB_2742808
LIVE/DEAD Fixable Aqua Dead Cell staining AmCyan	Invitrogen	Cat#L34957
Anti-human CD56 PE clone B159	BD Biosciences	Cat#555516; RRID:AB_395906
Anti-human CD107a V450 clone H4A3	BD Biosciences	Cat#561345; RRID:AB_10646032
Rabbit polyclonal anti-human IgG HRP	Dako	Cat#P021402-2
Goat anti-rabbit IgG HRP	Dako	Cat#P044801-2
Anti-human IFN-γ clone 1-D1K	Mabtech	Cat#3420-3-1000; RRID:AB_907282
Anti-human biotinylated IFN-γ clone 7-B6-1	Mabtech	Cat#3420-6-1000; RRID:AB_907272
Bacterial and virus strains
SARS-CoV-2 D614G primary isolate	This study	GISAID: hCoV-18/Netherlands/ZH-EMC-2498
SARS-CoV-2 Delta primary isolate	This study	GISAID: hCoV-19/Netherlands/NB-MVD-CWGS2201159
SARS-CoV-2 BA.1 primary isolate	This study	GISAID: hCoV-19/Netherlands/LI-SQD-01032/2022
Biological samples
Plasma	This study	N/A
PBMC	This study	N/A
Nanogram 100 mg/mL solution for infusion	Sanquin	Cat#RVG118226
Bovine serum albumin (BSA)	Sigma	Cat#A8327-50ML
Fetal bovine serum (FBS)	Merck Life Science	Cat#F7524-500ML
Horse serum	Merck Life Science	Cat#H1270-500ML
Human AB (hAB) serum; inactivated	Sigma	Cat#H6914
Chemicals, peptides, and recombinant proteins
SARS-CoV-2 Wu-Hu1 Spike Megapool	This study	N/A
SARS-CoV-2 Delta Spike Megapool	This study	N/A
SARS-CoV-2 BA.1 Spike Megapool	This study	N/A
DMSO	Honneywell	Cat#D5879-500ML
PMA	Merck Life Science	Cat#P1585-1MG
Ionomycin	Merck Life Science	Cat#I0634-5MG
Blocker blotto in TBS	Invitrogen	Cat#37530
Tween-20	Merck Life Science	Cat#P1379-1L
3,3′,5,5′-tetramethylbenzidine substrate	KPL	Cat#5120–0038
Sulfuric acid	Millipore	Cat#1.09073.1000
Lymphoprep	Stemcell Technologies	Cat#07861
Benzonase, purity grade 2	Millipore	Cat#1.01654.0001
SARS-CoV-2 biotinylated monomeric S protein D614G	Sino Biological	Cat#40589-V27B-B_100UG
SARS-CoV-2 trimeric S protein D614G	Sino Biological	Cat#40589-V08H6-100UG
SARS-CoV-2 trimeric S protein Delta	Sino Biological	Cat#40589-V08H10_100UG
SARS-CoV-2 trimeric S protein BA.1	Sino Biological	Cat#40589-V08H26_100UG
GolgiStop	BD Biosciences	Cat#554724
GolgiPlug	BD Biosciences	Cat#555029
Cytofix/Cytoperm	BD Biosciences	Cat#554722
Neutravidin FluoSphere beads	Life Technologies	Cat#F8775
Formaldehyde solution 37%	VWR	Cat#F1635-500ML
SARS-CoV-2 S1+S2 peptide pool	JPT Peptide Technologies	Cat#PM-WCPV-S
PHA	Remel Europe Ltd	Cat#HA16
Poly-HRP buffer	ThermoFisher	Cat#N500
Streptavidin poly-HRP	Sanquin	Cat#M2051
TMB substrate	Mabtech	Cat#2651–10
2-Mercaptoethanol	Merck Life Science	Cat#M3148-25ML
NaHCO3	Merck Life Science	Cat#S5761-500G
L-glutamine	Capricorn Scientific	Cat#GLN-B
Myo-inositol	Sigma	Cat#15125–50G
Folic acid	Sigma	Cat#F7876-25G
Sodium pyruvate 100mM	Gibco	Cat#11360–039
X-VIVO medium	Lonza	Cat#BE02-060F
Critical commercial assays
Liaison SARS-CoV-2 TrimericS IgG assay	DiaSorin	Cat#311510
QuantiFERON SARS-CoV-2 Blood Collection Tubes	Qiagen	Cat#626725
QuantiFERON ELISA	Qiagen	Cat#626410
DNA mini kit	Qiagen	Cat#51306
SARS-CoV-2 RBD B cell analysis kit	Miltenyi Biotec	Cat#130-128-022
immunoSEQ Assay	Adaptive Biotechnologies	N/A
Experimental models: Cell lines
NK92.05 – CD16	Kerry Campbell	Kind gift
THP-1	ATCC	Cat#TIB-202
Calu-3	ATCC	Cat#HTB-55
Software and algorithms
FlowJo v.10.8.1	TreeStar	N/A
PRISM v.9.4.1	GraphPad	N/A
Other
FACSLyric	BD Biosciences	N/A
Immunospot Imaging Analyzer	CTL Europe GmbH	N/A
ELISA microtiter plate reader Infinite F200	Tecan	N/A
FACS Canto II	BD Biosciences	N/A
SepMate-50 (IVD)	Stemcell Technologies	Cat#85460