Fig. 5.

FMT from D-gal-treated mice alleviated radiation-induced small intestine injury. (A) Schematic of the FMT assay. Mice were provided antibiotics in the drinking water for 4 weeks. Beginning on the day before irradiation, recipient mice were administered the microbiota mix from donor mice by oral gavage daily for 10 days. (B-C) Kaplan–Meier survival curve (B) and body weight (C) of mice in the FMT-saline and FMT-D-gal groups after the administration of 7.5 Gy of TBI; The number of mice in each group are as followed: Control: n = 37; FMT-Saline IR+: n = 36, P = 0.0015; FMT-D-gal IR+: n = 54, P < 0.0001. (D) Representative images of HE-stained small intestine sections from the indicated groups. Small intestines were collected on the seventh day after the administration of 13 Gy of TAI. Tissues were collected from at least 6 mice in each group. (E–I) The transcript levels of Glut1 (E), Mdr1 (F), Mus2 (G), Pgk1 (H) and Tff3 (I) in the small intestine of mice in different groups. The error bars in graphs means standard deviation of indicated data. ^*  P < 0.05, ^**  P < 0.01; n ≥ 7 mice per group. (J–K) IL-1β and IL-6 levels in intestinal homogenates measured using ELISA. The error bars in graphs means standard deviation of indicated data. ^*  P < 0.05, ^**  P < 0.01; n ≥ 7 mice per group. Data in (E)–(K) were analyzed for differences using independent samples t-test. Stars without a horizontal line were generated by comparing the IR-group in indicated treatment (E–I) or ‘Control IR-’ group (J and K). Stars with a horizontal line mean that the two groups were compared.