Table 1.
pIC50 and pEC50 Values for the effect of EGFR ligands on the binding of 14.6 nM Q44c-HL488 or 12.5 nM Q86c-HL488 to full-length N-terminal nanoluciferase-EGFR in HEK293 Cells.
| EGFR Ligand | Q44c-HL488 (pIC50) | Q86c-HL488 (pEC50) | EGF-AF488 (pKi) |
|---|---|---|---|
| EGF | 9.23 ± 0.11 (n=5) | 9.52 ± 0.06 (n=5) | 8.86 ± 0.07 (n=5) |
| Hb-EGF | 8.80 ± 0.13 (n=5) | 9.20 ± 0.17 (n=5) | 8.43 ± 0.08 (n=5) |
| TGF-α | 7.96 ± 0.19 (n=5) | 8.32 ± 0.09 (n=5) | 6.83 ± 0.05 (n=5) |
| BTC | 9.02 ± 0.14 (n=5) | 9.17 ± 0.09 (n=5) | 8.45 ± 0.05 (n=5) |
Values are mean ± S.E.M of n individual experiments.
These values have also been compared with their pKi values determined from inhibition of binding of 3 nM EGF-AF488.