Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 17;63(6):817–827. doi: 10.1093/jrr/rrac061

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022. Published by Oxford University Press on behalf of The Japanese Radiation Research Society and Japanese Society for Radiation Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Fig. 2 — Effects of emodin and radiation on apoptosis in GBM cells. (A) LN18 and LN428 cells were exposed to emodin (25 μM) and/or γ-ray (6 Gy) or neutron (6 GyE) radiation for 48 h for Annexin V staining. Values represent means of three experiments ± SD; ^*P < 0.05, ^*^*P < 0.01, ^*^*^*P < 0.001. (B) Analysis of cell death in two GBM cell lines 72 h after treatment with emodin using a cell death detection kit; ^*^*P < 0.01, ^*^*^*P < 0.001. (C) LN18 and LN428 cell was treated with γ-ray (6 Gy) or neutron (6 GyE) and/or emodin for 24 h. Cell cycle distribution (subG1) was analyzed quantitatively by flow cytometry; ^*P < 0.05. (D) TUNEL staining of LN18 and LN428 cells with and without neutron beam (6 GyE) or with and without emodin treatment. Values represent means of three experiments ± SD; ^*P < 0.05, ^*^*P < 0.01, ^*^*^*P < 0.001.

Fig. 2 — Effects of emodin and radiation on apoptosis in GBM cells. (A) LN18 and LN428 cells were exposed to emodin (25 μM) and/or γ-ray (6 Gy) or neutron (6 GyE) radiation for 48 h for Annexin V staining. Values represent means of three experiments ± SD; ^*P < 0.05, ^*^*P < 0.01, ^*^*^*P < 0.001. (B) Analysis of cell death in two GBM cell lines 72 h after treatment with emodin using a cell death detection kit; ^*^*P < 0.01, ^*^*^*P < 0.001. (C) LN18 and LN428 cell was treated with γ-ray (6 Gy) or neutron (6 GyE) and/or emodin for 24 h. Cell cycle distribution (subG1) was analyzed quantitatively by flow cytometry; ^*P < 0.05. (D) TUNEL staining of LN18 and LN428 cells with and without neutron beam (6 GyE) or with and without emodin treatment. Values represent means of three experiments ± SD; ^*P < 0.05, ^*^*P < 0.01, ^*^*^*P < 0.001.