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. 2022 Nov 23;13:1026780. doi: 10.3389/fmicb.2022.1026780

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Copyright © 2022 Choi, Heo and Chung.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Caffeine sensitivity of flr1 mutant is not recovered by Yap1 overexpression. (A,B) 10-fold serial dilutions of yeast mutant strains with YAP1 overexpression plasmids were spotted on SD/-URA media containing caffeine and incubated for 3 days at 30°C. (C) RT-PCR analysis of Yap1-dependent target genes expressed in WT, yap1 and YAP1 overexpression strains. ACT1 as a loading control. The products were analyzed by electrophoresis on a 1.5% agarose gel. The numbers of PCR cycles used are 22 (FLR1 and SOD1), 28 (YCF1 and ATR1) and 30 (ACT1), respectively.