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. 2022 Nov 23;13:1026780. doi: 10.3389/fmicb.2022.1026780

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Copyright © 2022 Choi, Heo and Chung.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Activation of Yap1 and its intracellular localization are not affected by caffeine treatment. (A) Fluorescent microscopy of the spontaneous or induced nuclear localization of Yap1-GFP in WT with H₂O₂ or caffeine treatment. White scale bar corresponds to 10 μm. (B) RT-PCR analysis of FLR1 and SOD1 expression in WT and yap1 mutant. ACT1 as a loading control. Cells were incubated at 30°C for 30 min with H₂O₂ or caffeine treatment. The numbers of PCR cycles used are 25 (SOD1) and 28 (FLR1 and ACT1), respectively.