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. 2022 Nov 23;13:1026780. doi: 10.3389/fmicb.2022.1026780

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Copyright © 2022 Choi, Heo and Chung.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ROS signaling modulates cellular adaptation to caffeine. (A) 10-fold serial dilutions of yeast mutant strains were spotted on SD/-URA media in the presence of caffeine with or without H₂O₂ and incubated for 3 days at 30°C. (B) Cell viability in the presence of caffeine was measured by counting CFUs on YEPD plates with or without H₂O₂. (C) 10-fold serial dilutions of yeast mutant strains were spotted on SD/-URA media in the presence of the caffeine with or without H₂O₂ and incubated for 3 days at 30°C.