Figure 4.

In Vitro serum neutralization of C. trachomatis SvD, SvE and SvF. Cynomolgus macaques were immunized following different prime-boost regimes (n = 5 per group) and serum was collected after finishing the immunization schedule (week 24). C. trachomatis SvD, SvE and SvF was incubated with serum from each animal and the capacity of the serum antibodies to inhibit infection were evaluated by incubating the antibody-bacteria mixture on to HaK cells and staining for inclusions. The black line shows SvD, the grey line shows SvE and the dark grey dotted line shows SvF. The dotted red line indicates the reciprocal 50% neutralization titer for each serovar.