Fig. 1.

Schematic representation of dCK enrichment procedure and assay of dCK activity. (a) The procedure involves the use of Q Sepharose ® anionic exchange beads to enrich dCK from cell lysates followed by concentration of the eluted fraction using Amicon® Ultra 0.5 ml Centrifugal Filters. Assessment of dCK activity is performed using the Kinase Glo ™ kit which measures the kinase activity indirectly by converting ATP left in the reaction mixture to light (luminescence) through luciferin/luciferase-dependent reaction. (b) Standard curve reflecting luciferase conversion of ATP. Different amount (µM) of ATP are converted into a sustained light output (λ = 570 nm), expressed as relative light units (RLUs), through a luciferin/luciferase-dependent reaction. dCK activity is inversely proportional to residual ATP concentration in the reaction medium