Table 1.
Antibody and fluorochrome panels to identify CLP and CMP from bone-marrow mononuclear cells
| Fluorochrome | ||||||
|---|---|---|---|---|---|---|
| PB * | PerCP* | PE* | APC* | AmCyan * | PE-Cy7* | |
| Antibody specificity | Lineage Cocktail (CD3, CD14, CD16, CD19, CD20, CD56) | CD10 | CD34 | CD38 | CD45RA | CD123 |
| Clone | SK7; 3G8; MφP9; SJ25C1; L27; NCAM16.2 | HI10a | 8G12 | HB-7 | HI100 | 9F5 |
| CLP | − | + | + | + | / | / |
| CMP | − | / | + | + | − | low |
+ and - signs mean positivity and negativity for a specific marker used for the gating strategy of a specific cell subset; / indicates a marker that is not used for the gating strategy
*PB Pacific blue, PerCP Peridinin Chlorophyll Protein Complex, PE phycoerythrin, APC Allophycocyanin, PE-Cy7 Peridinin chlorophyll protein-Cy5.5