FIG. 2.

In vitro expression and proteolytic activity in PVHSP28. The open reading frame of the PVHSP28 gene was used for in vitro expression with a rabbit reticulocyte lysate system. The translated products were immunoprecipitated with anti-Pv9 antibodies and analyzed by SDS-PAGE followed by autoradiography (A) as well as by gelatin-PAGE for zymography (B). The immunoprecipitates from the in vitro-translated products from a PVHSP28-encoding recombinant plasmid (lane 1) and vector alone (lane 2) were analyzed.