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Effect of metal ions on the P. vivax metalloprotease activitya
| Ion | Proteinase activity (%) in presence of 1 mM 1,10-phenanthrolineb
|
|
|---|---|---|
| Without ion | With ion (100 μM) | |
| ZnCl2 | 3.3 | 95.0 |
| CaCl2 | 1.5 | 53.0 |
| MgCl2 | 4.28 | 9.0 |
| MnCl2 | 5.4 | 78.0 |
Casein hydrolysis assay was used to determine the protease activity in the immunoprecipitate of the in vitro-translated product of the PVHSP gene.
The proteinase activity without 1,10-phenanthroline was considered 100% activity.
