Table 4.
Presence of genetically aberrant (aneusomic) cells in the different cellular compartments of (pre)malignant CIN lesions as detected by FISH targeting of chromosome 1 centromere (1C) copy numbers
| Histological classification | SOX2 pattern | Number of areasa | Size of aberrant area | (Para)basal cell layers | Intermediate cell layers | Superficial cell layers | |
|---|---|---|---|---|---|---|---|
| LSIL | CIN1 | 1 | 10 | Small | N | N | N |
| 2 | 1 | Small | A/ N | A/ N | N | ||
| HSIL | CIN1-2 | 1 | 3 | Small | A/ N | N | N |
| CIN2 | 1 | 14 | Small | A | A/ N | A/ N | |
| 2 | 3 | Medium | A | A | A/ N | ||
| CIN2-3 | 2 | 4 | Large | A | A | A/ N | |
| 3 | 4 | Large | N | A | A/ N | ||
| CIN3 | 2 | 9 | Large | A | A | A/ N | |
| 3 | 12 | Large | N | A | A/ N | ||
N Normal (Disomy; copy number for centromere of chromosome 1 (1C) = 2), A Aneusomy (copy number for centromere of chromosome 1 (1C) > 2), A/N Aneusomy/Normal (Aneusomic cells mixed with disomic cells). For a detailed description of the genetic classification, see the Supplemental Materials and Methods section. All histologically normal areas were genetically normal (disomic) and p16 negative. LSIL low-grade squamous intraepithelial lesion, HSIL high-grade squamous intraepithelial lesion
aNot all cases mentioned in Table 2 were available for FISH analysis. Only the number of analyzed areas is indicated.